Protective effect of HLA‐B57 on HCV genotype 2 infection in a West African population
Identifieur interne : 000C90 ( Main/Exploration ); précédent : 000C89; suivant : 000C91Protective effect of HLA‐B57 on HCV genotype 2 infection in a West African population
Auteurs : Wing Chia-Ming Chuang [Royaume-Uni] ; Francis Sarkodie [Ghana] ; Colin J. Brown [Royaume-Uni] ; Shirley Owusu-Ofori [Ghana] ; Juliette Brown [Royaume-Uni] ; Chengyao Li [Royaume-Uni] ; Cristina Navarrete [Royaume-Uni] ; Paul Klenerman [Royaume-Uni] ; Jean-Pierre Allain [Royaume-Uni]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2007-06.
English descriptors
- KwdEn :
- African americans, Allele, Allele frequency, Allelic frequency, Amino acids, Assay, Binding prediction programs, Blood donors, Candotti, Cellular, Cellular responses, Chronic hepatitis, Chronic infection, Chronic infections, Clear difference, Consensus sequences, Donor, Donor samples, Elispot, Elispot assay, Epitope, Genotype, Ghanaian, Ghanaian blood donors, Ghanaian donors, Ghanaian samples, Ghanaian subjects, Hepatitis, Hepatocellular carcinoma, High frequency, High rate, Host factors, Infection, Irish study, Killer cell receptor, National blood service, Negative control, Negative samples, Ns5b, Ns5b epitopes, Ns5b genotype, Ns5b sequence, Ns5b sequences, Odds ratio, Odds ratios, Overnight incubation, Pbmcs, Peripheral blood, Plasma samples, Present study, Protective effect, Rapid test, Reactive, Reactive band, Reactive samples, Recombinant, Recombinant core, Recombinant genotype, Recombinant proteins, Single source, Small number, Standard deviations, Strong association, Viral, Viral clearance, Viral load, Viral markers, Virol, Virus genotype, Virus infection, West africa, Western blot.
- Teeft :
- African americans, Allele, Allele frequency, Allelic frequency, Amino acids, Assay, Binding prediction programs, Blood donors, Candotti, Cellular, Cellular responses, Chronic hepatitis, Chronic infection, Chronic infections, Clear difference, Consensus sequences, Donor, Donor samples, Elispot, Elispot assay, Epitope, Genotype, Ghanaian, Ghanaian blood donors, Ghanaian donors, Ghanaian samples, Ghanaian subjects, Hepatitis, Hepatocellular carcinoma, High frequency, High rate, Host factors, Infection, Irish study, Killer cell receptor, National blood service, Negative control, Negative samples, Ns5b, Ns5b epitopes, Ns5b genotype, Ns5b sequence, Ns5b sequences, Odds ratio, Odds ratios, Overnight incubation, Pbmcs, Peripheral blood, Plasma samples, Present study, Protective effect, Rapid test, Reactive, Reactive band, Reactive samples, Recombinant, Recombinant core, Recombinant genotype, Recombinant proteins, Single source, Small number, Standard deviations, Strong association, Viral, Viral clearance, Viral load, Viral markers, Virol, Virus genotype, Virus infection, West africa, Western blot.
Abstract
Recovery from Hepatitis C virus (HCV) infection is considered infrequent (<20%) in western populations but reaches 50% in West Africa where genotype 2 infection is predominant. To investigate the role of cellular immune responses and host genetics in this phenomenon, samples from 104 Ghanaian blood donors reactive with anti‐HCV assays were collected between 2000 and 2005. HCV antibody was confirmed by Western blot using genotype 2 recombinant core, E2 and NS3 proteins. Viral load and genotype were determined. Samples were stratified into 37 chronic, 35 recovered infections and 32 false positive. Eighty‐one percentage of subjects with chronic infection (RNA positive) carried genotype 2 HCV. Cellular immune response was investigated in 35 frozen peripheral blood mononuclear cell (PBMC) samples suitable for interferon‐gamma ELISPOT assay. Twelve out of 24 confirmed recovered, 1 out of 5 chronically infected and none of the 6 false‐positive controls reacted to recombinant proteins. HLA‐A, ‐B and ‐DR types were determined by DNA methodology. HLA‐B*57 was significantly more frequent in the group which had recovered from HCV infection compared with chronically infected subjects (P = 0.0053, OR = 8.02). In conclusion, it is hypothesized that the dominance of genotype 2 HCV strains may be an important factor explaining the high rate of recovery from HCV infections in Ghana via an efficient contribution of HLA‐B*57 which is relatively frequent in the population. J. Med. Virol. 79: 724–733, 2007. © 2007 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20848
Affiliations:
- Ghana, Royaume-Uni
- Angleterre, Angleterre de l'Est, Grand Londres, Oxfordshire
- Cambridge, Londres, Oxford
- Université d'Oxford, Université de Cambridge
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Brown</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Histocompatibility & Immunogenetics, National Blood Service, Colindale, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Owusu Fori, Shirley" sort="Owusu Fori, Shirley" uniqKey="Owusu Fori S" first="Shirley" last="Owusu-Ofori">Shirley Owusu-Ofori</name><affiliation wicri:level="1"><country xml:lang="fr">Ghana</country><wicri:regionArea>Transfusion Medicine Unit, Department of Medicine, Komfo Anokye Teaching Hospital, Kumasi</wicri:regionArea><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Brown, Juliette" sort="Brown, Juliette" uniqKey="Brown J" first="Juliette" last="Brown">Juliette Brown</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Histocompatibility & Immunogenetics, National Blood Service, Colindale, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Li, Chengyao" sort="Li, Chengyao" uniqKey="Li C" first="Chengyao" last="Li">Chengyao Li</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>National Blood Service, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Navarrete, Cristina" sort="Navarrete, Cristina" uniqKey="Navarrete C" first="Cristina" last="Navarrete">Cristina Navarrete</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Histocompatibility & Immunogenetics, National Blood Service, 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xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Haematology, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country><wicri:regionArea>Division of Transfusion Medicine, Cambridge Blood Centre, Long Road, Cambridge CB2 2PT</wicri:regionArea><wicri:noRegion>Cambridge CB2 2PT</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">79</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="724">724</biblScope><biblScope unit="page" to="733">733</biblScope><biblScope unit="page-count">10</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2007-06">2007-06</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>African americans</term><term>Allele</term><term>Allele frequency</term><term>Allelic frequency</term><term>Amino acids</term><term>Assay</term><term>Binding prediction programs</term><term>Blood donors</term><term>Candotti</term><term>Cellular</term><term>Cellular responses</term><term>Chronic hepatitis</term><term>Chronic infection</term><term>Chronic infections</term><term>Clear difference</term><term>Consensus sequences</term><term>Donor</term><term>Donor samples</term><term>Elispot</term><term>Elispot assay</term><term>Epitope</term><term>Genotype</term><term>Ghanaian</term><term>Ghanaian blood donors</term><term>Ghanaian donors</term><term>Ghanaian samples</term><term>Ghanaian subjects</term><term>Hepatitis</term><term>Hepatocellular carcinoma</term><term>High frequency</term><term>High rate</term><term>Host factors</term><term>Infection</term><term>Irish study</term><term>Killer cell receptor</term><term>National blood service</term><term>Negative control</term><term>Negative samples</term><term>Ns5b</term><term>Ns5b epitopes</term><term>Ns5b genotype</term><term>Ns5b sequence</term><term>Ns5b sequences</term><term>Odds ratio</term><term>Odds ratios</term><term>Overnight incubation</term><term>Pbmcs</term><term>Peripheral blood</term><term>Plasma samples</term><term>Present study</term><term>Protective effect</term><term>Rapid test</term><term>Reactive</term><term>Reactive band</term><term>Reactive samples</term><term>Recombinant</term><term>Recombinant core</term><term>Recombinant genotype</term><term>Recombinant proteins</term><term>Single source</term><term>Small number</term><term>Standard deviations</term><term>Strong association</term><term>Viral</term><term>Viral clearance</term><term>Viral load</term><term>Viral markers</term><term>Virol</term><term>Virus genotype</term><term>Virus infection</term><term>West africa</term><term>Western blot</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>African americans</term><term>Allele</term><term>Allele frequency</term><term>Allelic frequency</term><term>Amino acids</term><term>Assay</term><term>Binding prediction programs</term><term>Blood donors</term><term>Candotti</term><term>Cellular</term><term>Cellular responses</term><term>Chronic hepatitis</term><term>Chronic infection</term><term>Chronic infections</term><term>Clear difference</term><term>Consensus sequences</term><term>Donor</term><term>Donor samples</term><term>Elispot</term><term>Elispot assay</term><term>Epitope</term><term>Genotype</term><term>Ghanaian</term><term>Ghanaian blood donors</term><term>Ghanaian donors</term><term>Ghanaian samples</term><term>Ghanaian subjects</term><term>Hepatitis</term><term>Hepatocellular carcinoma</term><term>High frequency</term><term>High rate</term><term>Host factors</term><term>Infection</term><term>Irish study</term><term>Killer cell receptor</term><term>National blood service</term><term>Negative control</term><term>Negative samples</term><term>Ns5b</term><term>Ns5b epitopes</term><term>Ns5b genotype</term><term>Ns5b sequence</term><term>Ns5b sequences</term><term>Odds ratio</term><term>Odds ratios</term><term>Overnight incubation</term><term>Pbmcs</term><term>Peripheral blood</term><term>Plasma samples</term><term>Present study</term><term>Protective effect</term><term>Rapid test</term><term>Reactive</term><term>Reactive band</term><term>Reactive samples</term><term>Recombinant</term><term>Recombinant core</term><term>Recombinant genotype</term><term>Recombinant proteins</term><term>Single source</term><term>Small number</term><term>Standard deviations</term><term>Strong association</term><term>Viral</term><term>Viral clearance</term><term>Viral load</term><term>Viral markers</term><term>Virol</term><term>Virus genotype</term><term>Virus infection</term><term>West africa</term><term>Western blot</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recovery from Hepatitis C virus (HCV) infection is considered infrequent (<20%) in western populations but reaches 50% in West Africa where genotype 2 infection is predominant. To investigate the role of cellular immune responses and host genetics in this phenomenon, samples from 104 Ghanaian blood donors reactive with anti‐HCV assays were collected between 2000 and 2005. HCV antibody was confirmed by Western blot using genotype 2 recombinant core, E2 and NS3 proteins. Viral load and genotype were determined. Samples were stratified into 37 chronic, 35 recovered infections and 32 false positive. Eighty‐one percentage of subjects with chronic infection (RNA positive) carried genotype 2 HCV. Cellular immune response was investigated in 35 frozen peripheral blood mononuclear cell (PBMC) samples suitable for interferon‐gamma ELISPOT assay. Twelve out of 24 confirmed recovered, 1 out of 5 chronically infected and none of the 6 false‐positive controls reacted to recombinant proteins. HLA‐A, ‐B and ‐DR types were determined by DNA methodology. HLA‐B*57 was significantly more frequent in the group which had recovered from HCV infection compared with chronically infected subjects (P = 0.0053, OR = 8.02). In conclusion, it is hypothesized that the dominance of genotype 2 HCV strains may be an important factor explaining the high rate of recovery from HCV infections in Ghana via an efficient contribution of HLA‐B*57 which is relatively frequent in the population. J. Med. Virol. 79: 724–733, 2007. © 2007 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Ghana</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Angleterre de l'Est</li><li>Grand Londres</li><li>Oxfordshire</li></region><settlement><li>Cambridge</li><li>Londres</li><li>Oxford</li></settlement><orgName><li>Université d'Oxford</li><li>Université de Cambridge</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Chuang, Wing Chia Ing" sort="Chuang, Wing Chia Ing" uniqKey="Chuang W" first="Wing Chia-Ming" last="Chuang">Wing Chia-Ming Chuang</name></region><name sortKey="Allain, Jean Ierre" sort="Allain, Jean Ierre" uniqKey="Allain J" first="Jean-Pierre" last="Allain">Jean-Pierre Allain</name><name sortKey="Allain, Jean Ierre" sort="Allain, Jean Ierre" uniqKey="Allain J" first="Jean-Pierre" last="Allain">Jean-Pierre Allain</name><name sortKey="Brown, Colin J" sort="Brown, Colin J" uniqKey="Brown C" first="Colin J." last="Brown">Colin J. Brown</name><name sortKey="Brown, Juliette" sort="Brown, Juliette" uniqKey="Brown J" first="Juliette" last="Brown">Juliette Brown</name><name sortKey="Klenerman, Paul" sort="Klenerman, Paul" uniqKey="Klenerman P" first="Paul" last="Klenerman">Paul Klenerman</name><name sortKey="Li, Chengyao" sort="Li, Chengyao" uniqKey="Li C" first="Chengyao" last="Li">Chengyao Li</name><name sortKey="Navarrete, Cristina" sort="Navarrete, Cristina" uniqKey="Navarrete C" first="Cristina" last="Navarrete">Cristina Navarrete</name></country><country name="Ghana"><noRegion><name sortKey="Sarkodie, Francis" sort="Sarkodie, Francis" uniqKey="Sarkodie F" first="Francis" last="Sarkodie">Francis Sarkodie</name></noRegion><name sortKey="Owusu Fori, Shirley" sort="Owusu Fori, Shirley" uniqKey="Owusu Fori S" first="Shirley" last="Owusu-Ofori">Shirley Owusu-Ofori</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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